Poster: Plant-pathogen interactions
Abs #
572: Proteomic analysis of rice defense response induced by probenazole
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Presenter: |
Lin, Yu-Zu , Lin0830@gate.sinica.edu.tw |
Authors | Lin, Yu-Zu (A) Kao, Ruby (A) Lai, Erh-Min (A) | | Affiliations: |
(A): Institute of Botany, Academia Sinica
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Systemic acquired resistance (SAR) is a potent innate immune system in plants. Salicylic acid (SA) is specifically identified as a signaling molecule inducing SAR development to against a broad spectrum of pathogens in tobacco and Arabidopsis. However, the roles of SA in rice resistance/defense mechanisms are disputed. The application of SA is not sufficient to induce the resistance/defense response in rice; in contrast, several synthetic chemicals are capable of inducing the SAR-like response in both Arabidopsis and rice. Thus, these effective chemicals would be useful for mimicking or investigating rice resistance/defense response. Here we utilize proteomics approach to reveal the protein expression profiles of rice seedlings in response to probenazole (PBZ), an effective SAR activator. The PBZ application has efficiently induced the resistance/defense response as the PBZ-treated rice seedlings are more resistant to bacterial blight pathogen Xanthomonas oryzae pv oryzae infection than the non-treated plants. Two-dimensional gel electrophoresis was utilized to resolve the proteins extracted from rice seedlings treated with and without PBZ respectively. The protein profiles obtained from PBZ-treated and non-treated rice seedlings at different time points were analyzed and classified by software ImageMasterTM 2D and GeneSpring to obtain the protein expression patterns with quantitative data. Several protein spots were detected to be consistently up or down-regulated by PBZ from at least three independent experiments. The differentially expressed spots were subjected to identification by mass spectrometry. The identities of PBZ-regulated protein spots will be presented and discussed for their biological significance in rice resistance/defense response.
