Poster: Regulation of gene expression

Abs # 757: Isolation, characterization and activity enhancement of rice rubi3 promoter

Presenter:	Sivamani, Elumalai , sivamanie@ncsu.edu
Authors	Sivamani, Elumalai  (A)   Qu, Rongda  (A)
Affiliations:	(A): North Carolina State University

A promoter from a rice polyubiquitin gene rubi3 has been isolated. The rubi3 gene contained an open reading frame of 1140 bp, arranged as five tandem, head to tail repeats of 228 bp, encoding a pentameric precursor ubiquitin gene. The 1.1 kb 5’ UTR intron of the gene strongly enhanced the promoter activity in transient expression assays. The activity of the rubi3 promoter was about half of the maize ubi1 promoter. In order to further enhance the activity of rubi3 promoter, three major modifications were made on this promoter. All the modifications resulted in a 3.5 - 6 fold increase in expression levels over wild type rubi3 promoter and 1.5 - 2.6 fold increase over the maize ubi1 promoter. Results of these experiments will be presented in the poster.