Poster: Regulation of gene expression

Abs # 758: Identification and characterization of genes in early seed development of rice

Presenter:	Kang, Sang Gu , kangsg@yu.ac.kr
Authors	Kang, Sang Gu  (A)   Jeong, Hey Kyeong  (A)   Lee, Dong Sun  (A)   Suh, Hak Soo  (B)
Affiliations:	(A): School of Biotechnology, Institute of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea (B): School of Biological Resouces, College of Natural Resources, Yeungnam University, Gyeongsan 712-749, Korea

Rice (Oryza sativa cv. Japonica group) genes involved in metabolic pathways and seed development were identified from the early flowers and the post-pollinated seeds. We performed the functional and molecular characterization for these identified cDNAs. By the functional anlaysis, some of the uncharacterized genes were chracterized as a novel glutathione peroxidase, a putative RING-H2, 3-isopropylmalate dehydratase, and novel eukaryotic lipoate-protein ligase. In addition, using the 60K rice whole oligomeric DNA microarray, we studied transcriptional profiles between the early meiosis and 5d after pollination specific stages in seed development. Large numbers of differentially expressed cDNAs in the two different developmental stages have been identified and discussed in their predicted functions for specific stages in developments. (This research was supported by a grant from Crop Functional Genomics Center, CG1213).