Poster: Regulation of gene expression
Abs #
782: Screening For Arabidopsis Mutants Affecting The Expression Of Soybean Seed Storage Proteins
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Presenter: |
Cui, Yuhai , cuiy@agr.gc.ca |
Authors | Hou, Anfu (A) Tang, Xurong (A) (B) Nguyen, Vi (A) Tsang, Ed W.T. (A) Cui, Yuhai (A) | | Affiliations: |
(A): Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre
(B): National Research Council Canada, Plant Biotechnology Institute
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| Web Site: | http://londr3/scpfrcweb/ | |
Soybean seed storage proteins are composed mainly (up to 70%) of glycinin (11S) and β–conglycinin (7S). The β–conglycinin contains three major subunits, α, α’ and β. These subunit genes are spatially and temporally regulated and coincide with seed development. Previously, promoter deletion analyses and cis element identification have been well documented. However, to our knowledge, little is known about the trans factors involved in the genetic regulations of soybean seed storage proteins. In an effort to improve our understanding of the genetic regulations of soybean storage proteins, we are conducting a genetic screen to search for trans factors involved in the control of soybean storage proteins, using Arabidopsis as a model plant. Soybean conglycinin promoter – GUS (β-glucuronidase) reporter chimeric genes have been constructed and introduced into Arabidopsis ecotype Columbia. Transgenic Arabidopsis plants have been produced. Seed GUS activity and GUS phenotype segregation patterns in the T2 generation have been analyzed. Homozygous lines for GUS at a single locus have been selected through generation segregations. These lines have shown stable and strong GUS activity and it appears that the regulation of these promoters is conserved in transgenic Arabidopsis plants in terms of temporal expression, tissue specificity, as well as hormone responsiveness. Seeds of homozygous lines have been subjected to EMS mutagenesis. One hundred and seventy one putative mutant plants have been identified which show altered GUS activity in seeds. Genetic analyses of these putative mutants are underway.
