Poster: Epigenetics & gene silencing

Abs # 827: Factors influencing the development of gene silencing within Agrobacteria infused tobacco leaves.

Presenter:	Velten, Jeff , jvelten@lbk.ars.usda.gov
Authors	Velten, Jeff  (A)   Cazzonelli, Christopher  (A)
Affiliations:	(A): usda-ars

A highly sensitive luciferase-based transient assay, combined with viral suppressors of silencing, has been used to examine factors influencing the induction and level of post transcriptional gene silencing (PTGS) within leaves of Nicotiana tobacum infused with Agrobacteria. The timing of PTGS development, as well as the influence on silencing of promoter strength, reporter coding region, and various environmental factors have been determined and compared with published findings in the alternative model plant, Nicotiana benthemiana.