Poster: Genome evolution
Abs #
838: Comparative analysis of unique Expressed Sequence Tags (ESTs) among Vitis species and their functional annotation against Arabidopsis genome
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Presenter: |
Lu, Jiang , jiang.lu@famu.edu |
Authors | Lu, Jiang (A) Huang, Hong (A) Hunter, Wayne (B) Dang, Phat (B) Leong, Stephen (A) | | Affiliations: |
(A): Florida A&M University
(B): USDA, ARS, Fort Pierce
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Vitis vinifera is a predominant grape species grown world wide for table consumption and processing for wine and other value added products. However, V. vinifera grape is very susceptible to diseases and pests. Vitis shuttleworthii is one of the most disease and pest resistant grape species originated in the southeast United States. Arabidopsis is the first plant genome being fully sequenced. To identify important genes related to viticulture, several labs have sequenced over 130,000 ESTs from various tissues of V. vinifera grapes including the most important wine grape cultivars ‘Chardonnay’, ‘Cabernet Sauvignon’ and ‘Saryh’ during last few years. In order to identify disease resistant and stress tolerant related genes that may not exist in V. vinifera grapes, our lab recently sequenced over 13,000 ESTs from a V. shuttleworthii grape cDNA library. A total of 12,008 single-pass ESTs were reduced to a set of 5,776 unique consensus sequences. A comparative study between V. vinifera, V. shuttleworthii ESTs and Arabidopsis genome will be helpful to understand putative genes and their functions in grapes. Over 1,400 of 5,766 species unique ESTs were identified when comparing Vitis shuttleworthii ESTs with 130,000 Vitis viniferia ESTs. The 1,400 Vitis shuttleworthii species unique ESTs set screening against the complete Arabidopsis genomic sequences (http://www.Arabidopsis.org) revealed that over 95% species unigenes have homologs in the Arabidopsis genome. finally 5,766 unigenes also are reannotated for their functionality against the Arabidopsis putative protein database. This information helps us to identify defense/disease resistant genes in grape.
