Poster: Genome evolution
Abs #
843: Construction of Genetic Linkage Map Based on Molecular Markers and Analysis of QTLs Controlling Morphological Traits in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
|
|
Presenter: |
Lim, Yong Pyo , yplim@cnu.ac.kr | Authors | Lim, Yong Pyo (A) Choi, Su Ryun (A) Kim, Jeong Hee (A) Piao, Zhong Yun (A) Plaha, Prikshit (A) Han, Tae Ho (B) Koo, Dal Hoe (C) Bang, Jae Wook (C) King, Graham J (D) Teakle, Graham R (D) Allender, Charlotte J (D) Lydiate, Derek J (E) Beynon, Elena (E) Osborn, Thomas C (F) | | Affiliations: |
(A): Department of Horticulture, College of Agiculture and Life Science
(B): Department of Applied Plant Science, Chonnam National University, Gwangju, 500-757, Korea
(C): Department of Biology, Chungnam National University, Daejeon, 305-764, Korea
(D): Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, UK
(E): Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N3V3, Canada
(F): University of Wisconsin, Madison WI
| |
|
A genetic linkage map of the Brassica rapa L. ssp.pekinensis was constructed based on a DNA markers such as AFLP, PCR-RFLP, ESTP, CAPs, SSR segregating in a doubled haploid population. This population was generated by crossing two morphologically diverse Chinese cabbage inbred lines, Chiifu and Kenshin. Out of the total 895 markers, 494 mapped on 10 linkage groups, covering 1,046 cM with an average distance of 2.1 cM. These markers were assigned to linkage groups of Chinese cabbage based on the SSR map of B. napus. Twenty one polygenic traits including yield and morphological attributes were studied for QTL analysis. Data recorded on these traits, based on replicated trials over years, were subjected to interval mapping using MapQTL versions 4.0. QTL with significant effect for head weight (16.70%), leaf blade width (37.40%), head compactness (32.50%) and head length (23.10%) were mapped on linkage groups LG1, LG7, LG2, and LG1, respectively. This map will serve as useful reference to undertake physical mapping and genome sequencing of B. rapa under the aegis of the Multinational Brassica Genome Project.
|
|
