Poster: Genome evolution
Abs #
851: Toward resolution of the fuzzy nodes in green plant phylogeny
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Presenter: |
Mandoli, Dina F., mandoli@u.washington.edu |
Authors | Mandoli, Dina F. (A) Olmstead, Richard (A) Mishler, Brent (B) Boore, Jeffrey (B) Smith, Alan (B) Renzaglia, Karen (D) Wolf, Paul (C) Donoghue, Michael (E) O'Kelley, Charley (F) | | Affiliations: |
(A): University Of Washington
(B): University of California Berkeley
(C): Utah State University
(D): Southern Illinois University at Carbondale
(E): Yale University
(F): Bigelow Laboratories for Ocean Sciences
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| Web Site: | http://faculty.washington.edu | |
We are funded to resolve the primary pattern of evolutionary diversification among green plants, and to establish a model for doing so that will be applicable to other groups of organisms with long evolutionary history. Evolution of organellar genomes will be a natural contribution from this work. To achieve this goal we will 1) complete a matrix of whole genome sequences for chloroplast and mitochondria and develop either nuclear or organellar bacterial artificial chromosome, BAC, libraries for about 50 representatives of the critical deep-branching lineages of green plants; 2) produce a comprehensive set of comparable morphological and ultrastructural data for these same taxa; 3) incorporate inferences from across the phylogenetic hierarchy in green plants using methods designed to permit scaling across studies. We shall indicate how this work will link to other research being conducted on green plants and various scales, especially the concatenation of our data sets with theirs. We are cloning organellar genomes from small amount of tissues (~ 40 mg) by coupling Fluorescent Activated Cell Sorting (FACS) of organelles with Rolling Circle Amplification (RCA) as well as from BAC libraries. Molecular and morphological data from genomes in progress will be presented. An overview of the analytical approaches will be shared. Funded by NSF's Tree of Life Program, 2002-2006, DEB #0228655 (lead institution), #228432, #0228679, #0228729, #0228660, #0228576.
