Poster: Genomic & proteomic resources
Abs #
867: Transcriptome Analysis of Heat Acclimation in Arabidopsis Suspension Cells
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Presenter: |
Lim, Chan Ju , mark-82@hanmail.net |
Authors | Lim, Chan Ju (A) Yang, Kyung Ae (A) Hong, Joon Ki (A) Bahk, Jeong Dong (A) Yun, Dae-Jin (A) Chung, Woo Sik (A) Lee, Sang Yeol (A) Lim, Chae Oh (A) | | Affiliations: |
(A): Division of Applied Life Science (BK21), Environmental Biotechnology Research Center, Gyeongsang National University
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Thermotolerance by mild heat acclimation is a genetically controlled manner in plants to overcome transiently temporal heat shock. Recently, this thermotolerance phenomenon have been extensively studied and focused on HSPs production. Despite these efforts and ubiquitous nature of the heat shock response, little is known how the plant senses an increase in temperature or signaling pathways resulting in HSPs. In this study, we report the heat acclimated Arabidopsis suspension cells show thermotolerance against 50กษ, 9 min, and their transcriptional profiles with cDNA microarrays for identifying thermotolerance involved genes. The optimal conditions showing thermotolerance were taken from using FDA staining, Chlorophyll assay, and Genomic DNA fragmentation test. A microarray technology containing 12288 cDNA clones was screened with cDNA samples taken at various times (0.5, 1, 2.5, 6, 16 h) after transfer to heat acclimation conditions. Through the time course of microarray experiments by heat acclimation, one third of the total genes were regulated in transcription level. They could be clustered in 11 up and/or down regulated groups. Among them, we could observed the expected strong expression of HSPs and HSFs. Interestingly, constantly induction of dehydration responsive DREB2B, KIN2, CBL1, etc were detected. In addition, TCA-elements, ABRE and TC-rich repeats were found from promotor analysis of coordinated induced gene. From these results, we could confirm the known heat stress signaling pathway, and suggest dehydration responsive pathways are participated in the heat stress defence mechanisms.
