Poster: Genomic & proteomic resources

Abs # 874: Proteome Dynamics during Cell Dedifferentiation Revealed Using Difference Gel Electrophoresis (DIGE) in Arabidopsis

Presenter:	Chitteti, Brahmananda Reddy , chnandu@yahoo.com
Authors	Chitteti, Brahmananda Reddy  (A)   Zhang, Lifeng  (A)   Peng, Zhaohua  (A)
Affiliations:	(A): mississippi state university

Cell dedifferentiation is a cell fate regression process in which the previous memory of cell fate is erased. The dedifferentiated cells regain the morphogenic and cell division potential, leading to the formation of new plants. In vivo cell dedifferentiation is related to wounding damage repair in both plants and animals. To investigate the process of cell dedifferentiation in Arabidopsis, the dynamic changes of the total proteome at different time points were examined using Fluorescence 2-D Difference Gel Electrophoresis (DIGE) approach. Among the 2423 protein spots analyzed, 1.8% of the proteins were up regulated more than two folds and 5.2% of the proteins were down regulated more than two folds. The proteins specifically regulated during cell dedifferentiation were identified using mass spectrometry analyses.