Poster: Genomic & proteomic resources

Abs # 886: Proteomic analysis of the Araidopsis thaliana vegetative vacuole

Presenter:	Raikhel, Natasha V, nraikhel@ucr.edu
Authors	Raikhel, Natasha V (A)   Carter, Clay  (A)   Pan, Songqin  (A)   Zouhar, Jan  (A)   Avila, Emily  (A)   Girke, Thomas  (A)
Affiliations:	(A): Center for Plant Cell Biology, Botany & Plant Sciences Dept., University of California, Riverside
Web Site:	http://cepceb.ucr.edu/members/raikhel.htm

Vacuoles are essential organelles for plant life. In order to better understand vacuolar function and biogenesis we have characterized the vegetative vacuolar proteome from Arabidopsis thaliana. Vacuoles were isolated from protoplasts derived from rosette leaf tissue. Total purified vacuolar proteins were subjected either to 2-dimensional liquid chromatography/tandem mass spectrometry or 1-D SDS PAGE followed by nano-liquid chromatography/tandem mass spectrometry (nano-LC MS/MS). A tonoplast-enriched fraction was also analyzed separately by 1-D SDS PAGE followed by nano-LC MS/MS. Cumulatively, a total of 381 proteins were identified from these analyses. An analysis of the identified proteins and their roles in vacuole function and biogenesis will be presented.