Poster: Genomic & proteomic resources
Abs #
887: Development of tools for the study of transcriptional function in Arabidopsis and tomato development: A two- component system for diverse regulation of transcription factor regulation.
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Presenter: |
Robert, Creelman A, bcreelman@mendelbio.com |
Authors | Zhang, James (A) Hempel, Fred (A) Adam, Luc (A) Palys, Joe (B) Robert, Creelman A (A) | | Affiliations: |
(A): Mendel Biotechnology, Inc.
(B): Seminis Inc.
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| Web Site: | http://www.mendelbio.com/ | |
Many important aspects of plant biology, such as fruit development and stress tolerance physiology, are regulated at transcriptional level. Mendel Biotechnology, Inc. has characterized Arabidopsis transcription factor (TF) function by phenotypic analysis of lines overexpressing or mutated for individual TFs. The function of many TFs has been well conserved evolutionarily (e.g., regulation of anthocyanin biosynthesis, seed development). In a collaboration with Seminis, we are in the process of evaluating the function of the entire complement of Arabidopsis TFs in tomato, with a focus on fruit biology, using a two-component system enabling the creation of lines in which each TF is under control of 10 different promoters. The objectives of this project are: 1) to determine the conservation of TF function in tomato with respect to function determiined in Arabidopsis; and 2) to identify TFs regulating fruit biology that were not readily studied in Arabidopsis. A preliminary field study was conducted in the summer of 2003 in which a sample of promoter-TF combinations for each of 250 TFs were studied. The system developed for this purpose will be described, and initial results validating the utility of the system in tomato and Arabidopsis will be presented. This system should be particularly useful for the elucidation of pathways involved in fruit development, and for the analysis of pathway evolution between tomato and Arabidopsis. One further aspect of the project is to evaluate the usefulness of tissue specific and developmental stage specific expression of TFs, in contrast to the standard 35S constitutive overexpression.
Research presented here funded by NIST Advanced Technology Program award 70NANB1H3004.
