Poster: Genomic & proteomic resources
Abs #
890: Proteomic Characterization of Herbicide Safener-induced Proteins in the Coleoptile of Triticum tauschii Seedlings
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Presenter: |
Zhang, Qin , qzhang@uiuc.edu |
Authors | Zhang, Qin (A) Riechers, Dean E. (A) | | Affiliations: |
(A): University of Illinois at Urbana-Champaign
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Proteomic methods such as 2D-PAGE and LC/MS/MS, as well as immunoblotting, were used to identify herbicide safener-induced proteins in the coleoptile of Triticum tauschii, a diploid wheat containing the D genome also found in hexaploid wheat Triticum aestivum. The herbicide safener fluxofenim dramatically increased protein abundance in the molecular weight range of 24 to 30 kDa, as well as a few higher molecular weight proteins, in the coleoptile of Triticum tauschii seedlings. In total, twenty proteins were identified in this study. Eleven proteins were highly safener induced and only weakly expressed in the control; seven proteins were new safener induced proteins that were not detected in the control. Two other proteins were constitutively expressed in both the control and safener-treated coleoptiles. Among the 18 inducible proteins, fifteen were glutathione S-transferase (GST) subunits that fall into three subclasses: eight proteins were from the tau subclass, six proteins were from the phi subclass, and one protein was from the lambda class. Another three safener inducible proteins showed homology with the aldo/keto reductase family and with proteins that have roles in glycolysis. Two constitutively expressed proteins were identified, one having highest homology with the dehydroascorbate reductase subclass of GSTs and one with an ascorbate peroxidase. Immunoblot analyses, using two different antisera raised against the same GST protein but differing in their specificity, were used to further characterize the GST proteins expressed in response to safener treatment. Results from immunoblotting, combined with mass spectral analysis, showed that post-translational modification of GST proteins in control and safener-treated coleoptiles may occur.
