Poster: Genomic & proteomic resources
Abs #
894: Making BAC libraries from algae, non-seed and seed plants: fundamental public resources for plant science
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Presenter: |
Mandoli, Dina F., mandoli@u.washington.edu |
Authors | Mandoli, Dina F. (A) Olmstead, Richard (A) Banks, Jody (B) dePamphilis, Claude (C) Carlson, John (C) Tomkins, Jeff (D) Luo, MeiZhong (E) Rao, Kiran (E) Kudrna, David (E) Wing, Rod (E) | | Affiliations: |
(A): University Of Washington
(B): Purdue University
(C): Pennsylvania State University
(D): Clemson University
(E): Arizona State University
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| Web Site: | http://faculty.washington.edu | |
The evolutionary transition to life on land and the diversification that followed required solving a series of problems such exposure to air and solar irradiation, life in a desiccating environment, etc. Plants solved these challenges with biochemical, cellular, anatomical and morphological innovations. We will provide access to the genetic basis of most of these innovations by making high-quality bacterial artificial chromosome, or BAC, libraries of 16 green plant species which are key in the evolutionary transition to land. These taxa include: Volvox carteri, Caulerpa taxifolia, Mesostigma viride, Coleochaete orbicularis, Chara sp., Marchantia polymorpha, Anthoceros sp., Selaginella moellendorfii, Angiopteris evecta, Ceratopteris richardii, Marsilea minuta, Amborella tricopoda, Nuphar advena, Acorus americana, Liriodendron tulipfera, Mimulus guttatus. All libraries have been or will be available to the public for a minimal cost via Clemson University Genome Institute or Arizona Genomics Institute as soon as they are made and characterized. Key innovations represented by each taxon and current research using these libraries from the community will be summarized. Most of the libraries will be available at the time of this meeting. We will describe our vouchering for these taxa, discuss biological issues we ran into in working with these taxa (e.g. many of the genome sizes in the literature differed dramatically from our values), describe how we are characterizing the libraries, and share technical roadblocks we have solved issues in making them. Funded by the NSF's First One Hundred Program, 2002-2005, IBN #0208502 (lead institution), #020211611, #0207110, and #0207202.
