Poster: Late and Moved Abstracts

Abs # 1033: Profiling temperature stress-responded gene expression in Vigna radiata by microarray analysis

Presenter:	Chen, Li-Ru , d908209@oz.nthu.edut.w
Authors	Lin, Tsai-Yun  (A)   Chen, Li-Ru  (A)   Tzeng, Rong-Xuan  (A)   Wang, Zhi-Giang  (A)
Affiliations:	(A): Department of Life Science and Institute of Bioinformatics and Structural Biology National Tsing Hua University
Web Site:	http://www.life.nthu.edu.tw/~lslty/

Temperature stress markedly eliminate crop productivity through the world. Plants can tolerate or adapt to the stress mediated multiple signaling pathways. Mungbean (Vigna radiata L.) is an important and economical legume in Asia area, and many cultivars have been bred by the Asian Vegetable Research and Development Center (AVRDC). Field observation indicated that mungbean cultivars NM94 and NM92 are more stress-tolerant than VC1973A. Employing the powerful tool of microarray, we aspire to clarify the strategy of stress tolerance hiding behind mungbean. The expressed sequence tags responded to temperature stress were assembled from substracted cDNA libraries of NM92, NM94 and VC1973A by exposing plants to different temperature. Using cDNA microarray, we profiled gene expression of mungbean responded to temperature stress during germination. We discriminated the significantly stress-responded EST using the Eisen Cluster and TreeView software. Moreover, we analyzed the expression pattern of stress genes using Northern hybridization. Substantially, evaluation of the stress genes, determination of their expression patterns in response to temperture stress, and an improved understanding of their roles in stress adaption will provide the basis of effective engineering strategies leading to greater stress tolerance.