Poster: Late and Moved Abstracts
Abs #
1001: Elicitation of Taxus meirei cell cultures for the improvement of Baccatin III production
Taxol is a potent chemotherapeutic drug with proven utility against a range of cancers. The supply of taxol cannot meet the demand for widening scope of administration and semisynthesis is currently an alternative way to obtain taxol. The technology of coupling the side chain with a suitably protected baccatin III to produce taxol shed the light on the use of baccatin III as a promising source for semisynthesis.
To increase taxol production in Taxus meirei cell suspensions, we cultured cells by feeding sugars, phenylalanine and methyl jasmonate and baccatin III production was observed. In this study, we attempted to improve baccatin III production with the addition of phenylalanine, methyl jasmonate, casein hydrolysate, or their combinations. The yields of baccatinIII reached 63.94 mg/L, 93.92 mg/L and 148.44 mg/L at 28 days after cultivation with 100 mM phenylalanine, 100 μ M methyl jasmonate and 0.1% casein hydrolysate, respectively, while that of control was 26.07 mg/L. The production of baccatin III was greatly enhanced when the combination was conducted. The baccatin III yields were 126.69 mg/L, 277.72 mg/L, 177.79mg/L, and 267.33mg/L for methyl jasmonate with phenylalanine, methyl jasmonate with casein hydrolysate, phenylalanine with methyl jasmonate, and methyl jasmonate with both of phenylalanine and casein hydrolysate, respectively. The highest yield (339.68 mg/L) of baccatin III was obtained at 56 days after cultivation by addition of methyl jasmonate and casein hydrolysate. This is the first report showing that the production of baccatin III was significantly enhanced by elicitation combined with casein hydrolysate, provided as a intermediate for semisynthesis to produce taxol and serve as a reliable and sustainable commercial source in the future.
