Poster: Late and Moved Abstracts
Abs #
1031: Expression and subcellular compartmentation of Aspergillus niger ß-glucosidase in transgenic tobacco increase insecticidal activity on whiteflies (Bemisia tabaci), and modulate plant growth, density of leaf secretory glandular trichomes and metabolic profiles
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Presenter: |
wei, shu , weishu@agri.huji.ac.il |
Authors | wei, shu (A) (B) Yaniv, Semel (A) Ben-Ami, Bravdo (A) Henryk, Czosnek (A) Oded, Shoseyov (A) | | Affiliations: |
(A): Hebrew University of jerusalem
(B): Horticulture Institute, Jiangsu Acadmey of Agricultural Sciences
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Transgenic tobacco plants expressing Aspergillus niger ß-glucosidase gene (BGL1) in different subcellular compartments [cell wall (Tcw), endoplasmic reticulum (Ter), and vacuole (Tvc)] were analyzed. Metabolic profiling indicated that 34 out of 56 compounds identified were significantly altered in transgenic plants: the majority decreased significantly in Tcw plants compared with wild-type and other transgenic plants. Hierarchical cluster analysis (HCA) showed that wild-type plants were closer to Tvc and Ter plants than to Tcw plants. Compared with wild-type controls, Ter and Tvc transgenic plants did not differ significantly in seed germination, plant growth rate, plant height, or flowering time. However, in Tcw plants seed germination and beginning of flowering were significantly delayed, and leaf area and plant fresh weight were significantly reduced. Transgenic plants had a marked insecticidal effect on whiteflies (Bemisia tabaci) as determined by caging insects with mature plants and by confining insects with detached leaves in closed vials. The density of secretory glandular trichomes was significantly greater in transgenic than in wild-type leaves. Our data indicate that expression of BGL1 in different subcellular compartments in transgenic tobacco may significantly alter metabolic pathways, growth, morphology and plant-insect interactions. This may shed a light on the biological role of ß-glucosides and ß-glucosidases in plant metabolism and plant insect interaction.
