Poster: Emerging technologies

Add this abstract to my Itinerary

Abs # 1031: New approaches of plant DNA repair signalling by non invasive imaging.

Presenter:	Chaboute, Marie-Edith , Contact Author
Authors	Roa, Helene  (A)   Keller, Murielle  (B)   Isac, Roxana  (A)   Houlne, Guy  (A)   Pale, Patrick  (B)   Grucker, Daniel  (C)   Montane, Marie-Helene  (D)   Chaboute, Marie-Edith  (A)
Affiliations:	(A): Institut de Biologie Moleculaire des Plantes, CNRS, ULP (B): Synthese, Biosynthese et Activite de Biomolecules, UMR 7123 (C): Laboratoire de neuroimagerie in vivo, UMR 7004 (D): CEA cadarache DSV-DEVM- Laboratoire de Radiobiologie Vegetale

Plants are continuously exposed to high levels of genotoxic stresses, especially to UV radiation, and therefore had to develop efficient DNA repair mechanisms. However, signalling pathways leading to DNA repair upon various genotoxic stresses remain poorly understood and begin only to be investigated in plants. Some key factors, such as ATM and ATR PI3 kinases, which play a major role in the DSB (DNA double strand break) and UV/ replicative stress pathways respectively, have been recently identified by sequence similarity to mammals. These factors target RNR genes and lead to their activation. RNR (Ribonucleotide reductase) is essential for providing the cell with the dNTP pool. Our goal is to analyze in time and space the expression profile in Arabidopsis of these RNR target genes following different genotoxic stresses. We have set up experimental conditions to analyze in vivo by MRI (Magnetic Resonance Imaging) various Arabidopsis mutants affected in DNA repair pathways. We are also developing tools for functional imaging by MRI, such as reporter molecules (caged paramagnetic molecules) specially designed for plant reporter genes. This totally new approach in plants will be presented, and the first results obtained discussed.