Minisymposium 7: Emerging Technologies

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Abs # M0703: Recombinomics: an analysis of whole genome recombination in Angiosperms and its use in predicting progeny sampling requirements during positional cloning

Presenter:	Raizada, Manish N       Contact Presenter
Authors	Raizada, Manish N (A)   Dinka, Steve  (A)   Barkin, Julian  (A)   Campbell, Matthew  (B)   Swarbreck, David  (C)
Affiliations:	(A): University of Guelph, Department of Plant Agriculture (B): The Institute for Genomic Research (C): The Arabidopsis Information Resource, Carnegie Institution of Washington, Department of Plant Biology
Web Site:	http://www.uoguelph.ca/plant/faculty/mraizada/index.html

We are interested in inter- and intra-species meiotic recombination frequencies in flowering plants, and the corresponding implications for gene mapping and positional cloning. As a fundamental question, we have measured the correlation between whole genome recombination and chromosome numbers in Eukaryotes. This data has implications for both evolution and positional cloning. Second, we have extensively analyzed positional cloning studies in Angiosperms. Based on this data, we have developed a simplified equation to help researchers predict how many F2 progeny must be sampled to achieve a particular candidate gene or kilobase resolution. Both the published studies and our model suggest that positional cloning is significantly more feasible in large crop genomes than previously predicted. We have then analyzed the recombination architecture along 2-5 cM segments of the rice and Arabidopsis genomes to help researchers calculate progeny sampling requirements along individual map intervals. Finally, we have calculated the feasibility of employing mapped, visible transgene reporters such as GFP to accelerate positional cloning efforts, a method we term Transgene Marker-Assisted Recombinant Selection (TMARS).