Poster: Environmental physiology

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Abs # P01003: Phosphatase activities and assimilation of organic phosphorus in Lolium multiflorum grown in vitro

Presenter:	Sharma, Nilesh C.       Contact Presenter
Authors	Sharma, Nilesh C. (A)   Paul, Pradyut K. (A)   Sahi, Shivendra V. (A)   Albano, Joseph P. (B)
Affiliations:	(A): Department of Biology, Western kentucky University (B): U.S. Horticultural Research Laboratory,USDA-ARS, 2001 S. Rock Rd, Ft. Pierce, FL 34945

Plants are known to have limited capacity to utilize organic P forms despite their abundance in soil. Gulf and Marshall ryegrass (Lolium multiflorum), which accumulate P in their shoots, were grown aseptically in agar-nutrient medium enriched with different organic P substrates (GMP, AMP, ATP and IHP) to study the pattern of growth, P accumulation and the activities of root phytase and phosphomonoesterase. Plants exhibited a differential pattern of growth in respect of the substrates, with minimum growth in the GMP- and maximum growth in the IHP- supplemented media. Plants accumulated P up to 1% (shoot dry weight) depending on the concentrations of IHP in media. The pattern of phytase and phosphomonoesterase activities in these grasses was interesting. The enzyme activities of plants grown in IHP-enriched media were either equal to or higher than controls that were grown in the media devoid of any P source. Experiments are in progress to compare the trends of growth and enzyme activities in these grass types with those of a related hybrid grass: Duo festulolium.