Poster: Environmental physiology

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Abs # P01009: Analysis of RSOsPR10, PR protein exclusively expressed in rice roots

Presenter:	Furukawa, Toshiko       Contact Presenter
Authors	Furukawa, Toshiko  (A)   Hashimoto, Makoto  (B)   Terakawa, Teruhiko  (C)   Okamoto, Takashi  (A)   Komatsu, Setsuko  (D)   Koshiba, Tomokazu  (A)
Affiliations:	(A): Tokyo Metropolitan University (B): Hitachi Science systems, Ltd. (C): Hokko Chemical Industry Co. Ltd. (D): National Institute of Agrobiological Sciences

RSOsPR10 gene expression (Root Specific Oryza sativa Pathogenesis-Related 10) is induced by drought and salt treatment in rice roots. This gene shows high similarity to PBZ1 gene, which codes a stress-induced PR 10 protein in rice. The mRNA was produced exclusively in the roots by drought, salt, probenazole, infection with rice blast fungus, and jasmonic acid (JA) (Hashimoto et al., PCP, 2004). These results implied the possibility that the gene was related to a defense response in rice roots. First, we analyzed the induction of RSOsPR10 protein in rice seedling with the specific antibody raised by recombinant RSOsPR10. As a result, an accumulation of this protein was found exclusively in the roots by salt, drought, wounding, and JA treatment, showing the same tendency as the mRNA patterns. Furthermore, the induction by salt, drought and JA treatment was almost completely suppressed by SA treatment (100μM). These results indicate that RSOsPR10 must be induced via JA and that JA and SA may act antagonistically. Next, we introduced the gene into rice (cv. Nipponbare) and obtained several transformant lines, which expressed RSOsPR10 protein continuously in the leaves and roots. We investigated the tolerance for NaCl and drought during seed germination and seedling growth. All the lines were more resistant to the stresses compared to wild type. We also made the antisense transformants. Some of them withered at the 3rd leaf stage and the seed mature rate was lower even in the case they grew to adults. These results indicate that RSOsPR10 protein must be related to abiotic stress tolerance in rice.