P66006: Effects of Magnetic Fields on Plant Growth and Development
Frederick, Cory M Contact Presenter||Authors||Frederick, Cory M (A) Wyatt, Sarah (A) |
(A): Department of Environmental and Plant Biology, Ohio University|
What effect does magnetism have on plant growth and development? The aquatic bacterium Magnetospirillum magnetotacticum biomineralize magnetite for migration to preferred oxygen gradients (magnetotaxis). The bacterial gene COG3536 is responsible for magnetosome development, iron oxide and iron sulfide nucleation, as well as nanocrystal synthesis. At3g27340, an Arabidopsis thaliana gene that encodes a protein of unknown function, has a similar protein sequence to COG3536. At3g27340 is located on the third chromosome of Arabidopsis thaliana, and two T-DNA insertion mutants, SALK_011817 and SALK_062099, have been selected for study. In initial experiments, two magnets (each 6.4mm x 24mm) were affixed to MS plates to create a negative field. Seeds of WT and mutants were plated either between the magnets in the low-G negative field or on plates without magnets, and the plates were clino-rotated. Clinorotation was employed because it eliminates the effects of gravity. After seven days, total seedling length was recorded along with hypocotyl bending. There was no significant difference among the control plants. In the clino-rotated groups, however SALK_011817 total seedling length averaged 8.96 mm, SALK_062099 averaged 4.55 mm, and WT averaged 4.95 mm. Reasons for the significant difference in growth rate for SALK_011817 are still being examined, and may be attributed in part to ferrous sulfate in the MS. Continued research includes the characterization and rescue of the phenotype of the mutants and a high-altitude flight to test the effect of solar radiation, gravity, response to the magnetic field and PT. Partial funding provided by ASPB SURF award and the Jeanette Graselli-Brown Undergraduate Research award.